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Hydrogels with time-dependent material properties enhance cardiomyocyte differentiation in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
8229 582 2011 8 PDF Available
Title
Hydrogels with time-dependent material properties enhance cardiomyocyte differentiation in vitro
Abstract

Tissue-specific elastic modulus (E), or ‘stiffness,’ arises from developmental changes in the extracellular matrix (ECM) and suggests that progenitor cell differentiation may be optimal when physical conditions mimic tissue progression. For cardiomyocytes, maturing from mesoderm to adult myocardium results in a 9-fold stiffening originating in part from a change in collagen expression and localization. To mimic this temporal stiffness change in vitro, thiolated-hyaluronic acid (HA) hydrogels were crosslinked with poly(ethylene glycol) diacrylate, and their dynamics were modulated by changing crosslinker molecular weight. With the hydrogel appropriately tuned to stiffen as heart muscle does during development, pre-cardiac cells grown on collagen-coated HA hydrogels exhibit a 3-fold increase in mature cardiac specific markers and form up to 60% more maturing muscle fibers than they do when grown on compliant but static polyacrylamide hydrogels over 2 weeks. Though ester hydrolysis does not substantially alter hydrogel stiffening over 2 weeks in vitro, model predictions indicate that ester hydrolysis will eventually degrade the material with additional time, implying that this hydrogel may be appropriate for in vivo applications where temporally changing material properties enhance cell maturation prior to its replacement with host tissue.

Keywords
Elastic modulus; Hyaluronic acid; Cardiomyocytes; Time-dependentHyaluronic Acid, (HA); Pascal, (Pa); poly(ethylene glycol) diacrylate, (PEGDA); degassed phosphate buffered saline, (DG PBS); atomic force microscopy, (AFM); nuclear magnetic resonance, (NMR)
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Hydrogels with time-dependent material properties enhance cardiomyocyte differentiation in vitro
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 4, February 2011, Pages 1002–1009
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us