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Gene delivery of PEI incorporating with functional block copolymer via non-covalent assembly strategy

Paper ID Volume ID Publish Year Pages File Format Full-Text
823 67 2013 10 PDF Available
Title
Gene delivery of PEI incorporating with functional block copolymer via non-covalent assembly strategy
Abstract

A novel functional diblock polymer P(PEGMA-b-MAH) is prepared and incorporated to improve the gene delivery efficiency of poly(ethyleneimine) PEI via non-covalent assembly strategy. First, P(PEGMA-b-MAH) is prepared from l-methacrylamidohistidine methyl ester (MAH) by reversible addition fragmentation chain transfer polymerization, with poly[poly(ethylene glycol) methyl ether methacrylate] (P(PEGMA)) as the macroinitiator. Then P(PEGMA-b-MAH) is assembled with plasmid DNA (pDNA) and PEI (Mw = 10 kDa) to form PEI/P(PEGMA-b-MAH)/pDNA ternary complexes. The agarose gel retardation assay shows that the presence of P(PEGMA-b-MAH) does not interfere with DNA condensation by the PEI. Dynamic light scattering tests show that PEI/P(PEGMA-b-MAH)/pDNA ternary complexes have excellent serum stability. In vitro transfection indicates that, compared to the P(PEGMA-b-MAH) free PEI-25k/pDNA binary complexes, PEI-10k/P(PEGMA-b-MAH)/pDNA ternary complexes have lower cytotoxicity and higher gene transfection efficiency, especially under serum conditions. The ternary complexes proposed here can inspire a new strategy for the development of gene and drug delivery vectors.

Graphical abstractP(PEGMA-b–MAH) is assembled with pDNA and PEI-10k to form PEI/(PEGMA-b–MAH)/pDNA ternary complexes with desirable stability and high transfection efficiency.Figure optionsDownload full-size imageDownload high-quality image (107 K)Download as PowerPoint slide

Keywords
Poly(ethyleneimine); Functional diblock copolymer; Ternary complexes; Gene vector
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Gene delivery of PEI incorporating with functional block copolymer via non-covalent assembly strategy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 9, Issue 2, February 2013, Pages 5003–5012
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us