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The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function

Paper ID Volume ID Publish Year Pages File Format Full-Text
8273 584 2011 8 PDF Available
Title
The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function
Abstract

The aim of this study was to investigate biologic function of bone morphorgenic protein-2 (rhBMP-2) immobilized on the heparin-grafted Ti surface. Ti surfaces were first modified by 3-aminopropyltriethoxysilane (ATPES), followed by grafting of heparin. BMP-2 was then immobilized on the heparin-grafted Ti surfaces. Pristine Ti and functionalized Ti surfaces were characterized by X-ray photoelectron spectroscopy (XPS), measurement of water contact angles, and protein adsorption. The biological activity of MG-63 cells on pristine and functionalized Ti surfaces was investigated by cell proliferation assays, measurement of alkaline phosphate (ALP) activity, and determination of calcium deposition. Anti-inflammatory effects were assessed by RT-PCR to measure the transcript levels of IL-6 and TNF-α. XPS revealed that heparin and BMP-2 were successfully grafted and immobilized on the Ti surfaces, respectively. In addition, Ti surfaces with BMP-2 immobilized were more hydrophilic than pristine Ti. Furthermore, BMP-2 immobilized Ti promoted significantly higher ALP activity and calcium deposition by MG-63 cells than pristine Ti. The inflammatory response was also decreased when cells were grown on heparin-grafted, BMP-2-immobilized Ti surfaces. The results of this study suggest that by grafting heparin and immobilizing BMP-2 on Ti surfaces, inflammation can be inhibited and osteoblast function promoted.

Keywords
Titanium; Heparin; Bone morphogenic protein-2 (BMP-2); Osteoblasts; Inflammation; Peri-implantitis
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The effect of immobilization of heparin and bone morphogenic protein-2 (BMP-2) to titanium surfaces on inflammation and osteoblast function
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 2, January 2011, Pages 366–373
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us