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Low molecular weight alkyl-polycation wrapped magnetite nanoparticle clusters as MRI probes for stem cell labeling and in vivo imaging

Paper ID Volume ID Publish Year Pages File Format Full-Text
8289 584 2011 10 PDF Available
Title
Low molecular weight alkyl-polycation wrapped magnetite nanoparticle clusters as MRI probes for stem cell labeling and in vivo imaging
Abstract

Superparamagnetic iron oxide (SPIO) nanoparticles are potential probes for noninvasive cell tracking, but the design of safe probes coupled with high labeling efficiency is still an important objective for such application. In this study, an efficient SPIO probe has been developed for mesenchymal stem cells (MSCs) labeling and tracking. Different from many other systems involving high molecular polycations, we chose low molecular weight amphiphilic PEI2k to form stable nanocomplexes with SPIO nanoparticles. The probe can hold multiple SPIO nanoparticles with a controlled clustering structure, leading to much higher T2 relaxivities compared to single SPIO nanoparticles. Labeled MSCs are unaffected in their viability, proliferation, or differentiation capacity. The iron uptake process in MSCs displays a time- and dose-dependent behavior. Transmission electron microscopy reveals that the nanoprobes are internalized into the cytoplasm of MSCs. Subcutaneous injection of the labeled MSCs dispersed in a collagen type I hydrogel showed strong image contrast against unlabeled cells under a clinical 3T magnetic resonance imaging (MRI) scanner up to 19 days post-transplantation. This study provides an important alternative to label MSCs at optimized low dosages with high efficiency, and the probe may be useful to label other biologically important cells for imaging studies.

Keywords
Superparamagnetic iron oxide; Controlled clustering; Mesenchymal stem cells; Magnetic resonance imaging; Cell tracking
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Low molecular weight alkyl-polycation wrapped magnetite nanoparticle clusters as MRI probes for stem cell labeling and in vivo imaging
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 2, January 2011, Pages 528–537
Authors
, , , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us