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Modeling receptor-mediated endocytosis of polymer-functionalized iron oxide nanoparticles by human macrophages

Paper ID Volume ID Publish Year Pages File Format Full-Text
8291 584 2011 9 PDF Available
Title
Modeling receptor-mediated endocytosis of polymer-functionalized iron oxide nanoparticles by human macrophages
Abstract

Although systemically applied nanoparticles are quickly taken up by phagocytic cells, mainly macrophages, the interactions between engineered nanoparticles and macrophages are still not well defined. We therefore analyzed the uptake of diagnostically used carboxydextran-coated superparamagnetic iron oxide nanoparticles of 60 nm (SPIO) and 20 nm (USPIO) by human macrophages. By pharmacological and in vitro knockdown approaches, the principal uptake mechanism for both particles was identified as clathrin-mediated, scavenger receptor A-dependent endocytosis. We developed a mathematical model of the uptake process that allows determination of key parameters of endocytosis, including the rate of uptake, the number of nanoparticles per cell in saturation, the mean uptake time, and the correlation between the number of internalized nanoparticles and their extracellular concentration. The calculated parameters correlate well with experimental data obtained by confocal microscopy. Moreover, the model predicts the individual and collective wrapping times of different nanoparticles, describes the relation between cytoskeletal forces, membrane elasticity and the uptake time. We also introduced a new physical parameter ‘a’ governing the collective uptake process, a reflecting minimal linear spacing between simultaneously acting neighboring endocytotic pits.

Keywords
Macrophage; Nanoparticle; Bioabsorption; Modeling; Antisense; MRI (magnetic resonance imaging)
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Modeling receptor-mediated endocytosis of polymer-functionalized iron oxide nanoparticles by human macrophages
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 2, January 2011, Pages 547–555
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us