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Multilayered silk scaffolds for meniscus tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
8300 584 2011 13 PDF Available
Title
Multilayered silk scaffolds for meniscus tissue engineering
Abstract

Removal of injured/damaged meniscus, a vital fibrocartilaginous load-bearing tissue, impairs normal knee function and predisposes patients to osteoarthritis. Meniscus tissue engineering solution is one option to improve outcomes and relieve pain. In an attempt to fabricate knee meniscus grafts three layered wedge shaped silk meniscal scaffold system was engineered to mimic native meniscus architecture. The scaffolds were seeded with human fibroblasts (outside) and chondrocytes (inside) in a spatial separated mode similar to native tissue, in order to generate meniscus-like tissue in vitro. In chondrogenic culture in the presence of TGF-b3, cell-seeded constructs increased in cellularity and extracellular matrix (ECM) content. Histology and Immunohistochemistry confirmed maintenance of chondrocytic phenotype with higher levels of sulfated glycosaminoglycans (sGAG) and collagen types I and II. Improved scaffold mechanical properties along with ECM alignment with time in culture suggest this multiporous silk construct as a useful micro-patterned template for directed tissue growth with respect to form and function of meniscus-like tissue.

Keywords
Silk; Meniscus; Cartilage; Tissue engineering; Biomaterials
First Page Preview
Multilayered silk scaffolds for meniscus tissue engineering
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 2, January 2011, Pages 639–651
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering