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The precise control of cell labelling with streptavidin paramagnetic particles

Paper ID Volume ID Publish Year Pages File Format Full-Text
8310 585 2009 8 PDF Available
Title
The precise control of cell labelling with streptavidin paramagnetic particles
Abstract

A previously developed cell labelling methodology has been evaluated to assess its potential to precisely control the degree of magnetic labelling. The two-step method provides a quick way of labelling cells by first biotinylating the cell membrane proteins and then binding streptavidin paramagnetic particles onto the biotinylated proteins. Characterisation studies on biotinylated HeLa cells have revealed that the biotin concentration on the cell surface can be varied by changing the biotinylating reagent concentration. At the optimal concentration (750 μm), a substantial surface biotin density (∼108 biotin per cell) could be achieved within 30 min. The degree of magnetic labelling could be altered by adjusting the concentration of paramagnetic particles added to the cells and the binding of the particles onto the cell surface was not considerably affected by the biotin density on the cell surface. The magnetic moment of the labelled cells was measured and correlated well with the degree of magnetic labelling. Cell viability studies indicated that the magnetic labelling was not cytotoxic. Magnetically labelled cells were then successfully targeted and manipulated by magnetic fields to form three dimensional multicellular structures.

Keywords
Magnetic cell labelling; Biotin; Streptavidin; Paramagnetic particles; Cell targeting; Cell manipulation
First Page Preview
The precise control of cell labelling with streptavidin paramagnetic particles
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 33, November 2009, Pages 6548–6555
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering