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In situ forming collagen–hyaluronic acid membrane structures: Mechanism of self-assembly and applications in regenerative medicine

Paper ID Volume ID Publish Year Pages File Format Full-Text
838 67 2013 9 PDF Available
Title
In situ forming collagen–hyaluronic acid membrane structures: Mechanism of self-assembly and applications in regenerative medicine
Abstract

Bioactive, in situ forming materials have the potential to complement minimally invasive surgical procedures and enhance tissue healing. For such biomaterials to be adopted in the clinic, they must be cost-effective, easily handled by the surgeon and have a history of biocompatibility. To this end, we report a novel and facile self-assembling strategy to create membranes and encapsulating structures using collagen and hyaluronic acid (HA). Unlike membranes built by layer-by-layer deposition of oppositely charged biomolecules, the collagen–HA membranes described here form a diffusion barrier upon electrostatic interaction of the oppositely charged biomolecules, which is further driven by osmotic pressure imbalances. The resulting membranes have a nanofibrous architecture, a thicknesses of 130 μm and a tensile modulus (0.59 ± 0.06 MPa) that can increase 7-fold using carbodiimide chemistry (4.42 ± 1.46 MPa). Collagen–HA membranes support mesenchymal stem cell proliferation and have a slow and steady protein release profile (7% at day 28), offering opportunities for targeted tissue regeneration. We demonstrate the capacity to encapsulate cells by injecting HA into the collagen solution, and enhance allograft and implant biocompatibility through a coating technique. This study describes a novel mechanism of collagen–HA membrane formation and provides the groundwork to apply these membranes in a variety of tissue engineering applications.

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Keywords
Self-assembly; Membrane; Collagen; Cell encapsulation; Bioplotting
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In situ forming collagen–hyaluronic acid membrane structures: Mechanism of self-assembly and applications in regenerative medicine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 9, Issue 2, February 2013, Pages 5153–5161
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us