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The enhancement of endothelial cell therapy for angiogenesis in hindlimb ischemia using hyaluronan

Paper ID Volume ID Publish Year Pages File Format Full-Text
8402 589 2011 12 PDF Available
Title
The enhancement of endothelial cell therapy for angiogenesis in hindlimb ischemia using hyaluronan
Abstract

Growing evidence shows that injection of hyaluronan (HA) benefits ischemic injury in animals. On the other hand, cell therapy is an emerging approach to treat occlusive arterial diseases, although the low retention rate of cells after direct injection remains a major concern. Here, we tested whether injection of HA along with endothelial cells promotes the retention and growth of transplanted cells, thus improving therapeutic angiogenesis in a mouse model of hindlimb ischemia (HI). In culture, HA improved human umbilical vein endothelial cell (HUVEC) proliferation proportional to HA concentration and protected HUVECs from apoptosis. Subsequently, in immunocompromised mice HI was induced by femoral artery ligation and treatments were given 24 h later. At 4 weeks, injection of HA along with HUVECs had a greater effect for restoring blood perfusion and salvaging the ischemic limb compared to injection of HA or HUVECs alone. In addition, angiogenesis and arteriogenesis were significantly increased by HA + HUVECs injection. Lastly, HA + HUVECs injection resulted in the retention of more cells than HUVECs alone, and allowed their engraftment into the vasculature of the ischemic limb. These results suggest that this combined approach can be translated into a clinical therapy for peripheral artery occlusive disease.

Keywords
Angiogenesis; Endothelial cell; Hyaluronan; Peripheral artery occlusive disease
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The enhancement of endothelial cell therapy for angiogenesis in hindlimb ischemia using hyaluronan
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 1, January 2011, Pages 75–86
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us