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Effect of ProNectin F derivatives on cell attachment and proliferation

Paper ID Volume ID Publish Year Pages File Format Full-Text
842 67 2013 7 PDF Available
Title
Effect of ProNectin F derivatives on cell attachment and proliferation
Abstract

ProNectin F (PnF) was chemically modified by introducing some functional groups to prepare various derivatives of primary amino (PnF-N1), tertiary amino (PnF-N3), quaternary ammonium (PnF-N4), carboxyl (PnF-COOH) and sulfonyl groups (PnF-SO3H). When C3H10T1/2 cells were cultured on non-treated dishes coated with the derivatives, the number of mesenchymal cells attached to the culture dishes increased for the coating with PnF-COOH and PnF-SO3H, even at their low adsorption amount. The cytotoxicity was high for the coating of PnF-N1 and PnF-N4 compared with that of the PnF-N3, PnF-COOH and PnF-SO3H. The treatment with integrin α5 and αV antibodies suppressed the cell attachment to the dishes coated with PnF-COOH and PnF-SO3H. The phosphorylation of extracellular signal-regulated kinase (ERK) was upregulated for cells attached to the dishes coated with PnF-COOH and PnF-SO3H, indicating their enhanced proliferation. It is concluded that the chemical derivatization of PnF enhanced the ability of cell attachment and proliferation.

Keywords
ProNectin F; Artificial protein; Cell adhesion; Proliferation; Integrin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 9, Issue 2, February 2013, Pages 5194–5200
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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