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The proliferation and differentiation of placental-derived multipotent cells into smooth muscle cells on fibrillar collagen

Paper ID Volume ID Publish Year Pages File Format Full-Text
8443 590 2010 9 PDF Available
Title
The proliferation and differentiation of placental-derived multipotent cells into smooth muscle cells on fibrillar collagen
Abstract

Type I collagen constitutes a major portion of the extracellular matrix (ECM) in arterial wall and it is the major substrate for cell growth and differentiation. The goal of this study was to evaluate the differentiation and proliferation of placenta-derived multipotent cells (PDMCs) on polymerized type I collagen fibrils and monomer collagen. PDMCs grown on both polymerized collagen and monomer collagen with transforming growth factor (TGF)-β treatment increases the expression of smooth muscle cell (SMC)-specific markers, including calponin, α-smooth muscle actin (α-SMA) and smooth muscle-myosin heavy chain (SM-MHC). Polymerized collagen increased the expressions of p21CIP1 and p27KIP1; decreased cyclin A, cyclin D1, cyclin-dependent protein kinase 2 (Cdk2); and led to G0/G1 arrest in PDMCs. Furthermore, PDMC–differentiated SMCs exhibited significant collagen contractility in the presence or absence of endothelin-1 (ET-1) stimulation. By using specific inhibitors and small interfering RNA (siRNA), we demonstrated that p38 MAPK pathway and serum response factor (SRF)–DNA binding activity is critical for the polymerized collagen-induced PDMC differentiation into SMCs. Thus, polymerized collagen exhibits the great potential in inducing PDMCs differentiation into SMCs, and exerts anti-proliferative effect on PDMC–differentiated SMCs.

Keywords
Placenta-derived multipotent cells; Smooth muscle cells; Type I collagen; Differentiation; p38 mitogen-activated protein kinase
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The proliferation and differentiation of placental-derived multipotent cells into smooth muscle cells on fibrillar collagen
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 15, May 2010, Pages 4367–4375
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us