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Honeybee silk: Recombinant protein production, assembly and fiber spinning

Paper ID Volume ID Publish Year Pages File Format Full-Text
8480 591 2010 6 PDF Available
Title
Honeybee silk: Recombinant protein production, assembly and fiber spinning
Abstract

Transgenic production of silkworm and spider silks as biomaterials has posed intrinsic problems due to the large size and repetitive nature of the silk proteins. In contrast the silk of honeybees (Apis mellifera) is composed of a family of four small and non-repetitive fibrous proteins. We report recombinant production and purification of the four full-length unmodified honeybee silk proteins in Escherichia coli at substantial yields of 0.2–2.5 g/L. Under the correct conditions the recombinant proteins self-assembled to reproduce the native coiled coil structure. Using a simple biomimetic spinning system we could fabricate recombinant silk fibers that replicated the tensile strength of the native material.

Keywords
Biomimetic material; FTIR; Honeybee; Apis mellifera; Recombinant protein; Silk
First Page Preview
Honeybee silk: Recombinant protein production, assembly and fiber spinning
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 9, March 2010, Pages 2695–2700
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering