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The use of phospholipase A2 to prepare acellular porcine corneal stroma as a tissue engineering scaffold

Paper ID Volume ID Publish Year Pages File Format Full-Text
8483 592 2009 10 PDF Available
Title
The use of phospholipase A2 to prepare acellular porcine corneal stroma as a tissue engineering scaffold
Abstract

This study was to develop a method using phospholipase A2 (PLA2) to prepare acellular porcine corneal stroma (APCS) for tissue engineering. The APCS was prepared from native porcine cornea (NPC) that was treated with 200 U/ml PLA2 and 0.5% sodium deoxycholate (SD). The removal of DNA content, representing decellularization efficiency, reached to 91%, while all hydroxyproline and 80% of glycosaminoglycan were retained in the APCS when compared with NPC. The residual PLA2 and SD were 0.35 ± 0.04 U/mg dry weight and 4.3 ± 0.8 ng/mg dry weight respectively. The extracts of APCS had no inhibitory effects on proliferation of corneal epithelial and endothelial cells as well as keratocytes. There was no sign of infiltration of neutrophilic leukocytes or leukomonocytes at 2 weeks after subcutaneous implantation of APCS. The prepared APCS displayed similar light transmittance to NPC. There were no significant differences in the areal modulus and curvature variation between APCS and NPC. Rabbit lamellar keratoplasty showed that the grafts of APCS were epithelialized completely in 8 ± 2 days, and their transparency was restored in 84 ± 11 days when the light transmittance of APCS-transplanted corneas displayed no significant difference compared with native corneas. Corneal neovascularization, corneal deformation, and graft degradation were not observed within 12 months.

Keywords
Corneal tissue engineering; Scaffold; Phospholipase A2; Sodium deoxycholate; Decellularization
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The use of phospholipase A2 to prepare acellular porcine corneal stroma as a tissue engineering scaffold
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 21, July 2009, Pages 3513–3522
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us