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Electrochemical desorption of self-assembled monolayers for engineering cellular tissues

Paper ID Volume ID Publish Year Pages File Format Full-Text
8490 592 2009 7 PDF Available
Title
Electrochemical desorption of self-assembled monolayers for engineering cellular tissues
Abstract

Adherent cells, cell sheets, and spheroids were harvested noninvasively from a culture surface by means of electrochemical desorption of a self-assembled monolayer (SAM) of alkanethiol. The SAM surface was made adhesive by the covalent bonding of Arg-Gly-Asp (RGD)-peptides to the alkanethiol molecules. The application of a negative electrical potential caused the reductive desorption of the SAM, resulting in the detachment of the cells. Using this approach greater than 90% of adherent cells detached within 5 min. Furthermore, this approach was used to obtain two-dimensional (2D) cell sheets. The detached cell sheets consisted of viable cells, which could be easily attached to other cell sheets in succession to form a multilayered cell sheet. Moreover, spheroids of hepatocytes of a uniform diameter were formed in an array of cylindrical cavities at a density of 280 spheroids/cm2 and were harvested by applying a negative electrical potential. This cell manipulation technology could potentially be a useful tool for the fabrication and assembly of building blocks such as cell sheets and spheroids for regenerative medicine and tissue engineering applications.

Keywords
Cell sheet; Spheroid; Fibroblast; Hepatocyte; Self-assembled monolayer; Electrochemistry
First Page Preview
Electrochemical desorption of self-assembled monolayers for engineering cellular tissues
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 21, July 2009, Pages 3573–3579
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering