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Creating gradients of two proteins by differential passive adsorption onto a PEG-density gradient

Paper ID Volume ID Publish Year Pages File Format Full-Text
8499 595 2010 6 PDF Available
Title
Creating gradients of two proteins by differential passive adsorption onto a PEG-density gradient
Abstract

Many fundamental biological processes, including early embryo development, immune responses and the progression of pathogens, are mediated by gradients of biological molecules. Understanding these vital physiological processes requires the development of biomaterial platforms that can mimic them in-vitro. Such platforms include laboratory generated surface gradients of biological molecules. In this work, we report a method for the generation of surface gradients of two proteins. We used a surface grafting density gradient of polyethylene glycol (PEG) to control protein adsorption. In addition, we used protein size as a tool to control the position and the adsorbed amount of both proteins. To demonstrate our concept, we used fibrinogen as an example of a large protein and lysozyme as an example of a small protein. However, we speculate that the same strategy could be extended to any other pair of large and small proteins. We used X-ray photoelectron spectroscopy and sessile drop contact angle measurements to determine the chemical composition and wettability of the gradients. Protein adsorption was studied by surface plasmon resonance imaging.

Keywords
PEG gradient; Protein gradients; Protein adsorption; Plasma deposition; Surface grafting
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Creating gradients of two proteins by differential passive adsorption onto a PEG-density gradient
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 3, January 2010, Pages 392–397
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us