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Redox-cleavable star cationic PDMAEMA by arm-first approach of ATRP as a nonviral vector for gene delivery

Paper ID Volume ID Publish Year Pages File Format Full-Text
8517 595 2010 11 PDF Available
Title
Redox-cleavable star cationic PDMAEMA by arm-first approach of ATRP as a nonviral vector for gene delivery
Abstract

In this work, we synthesized a star cationic polymer(s-PDMAEMA) consisting of cleavable poly[N,N-bis(acryloyl) cystamine](PBAC) crosslinked core and poly(N,N-dimethyl-ethylamine methacrylate) (PDMAEMA) arms by atomic transfer radical polymerization using one-pot “arm first” method. The s-PDMAEMA that was degradable in a mimic intracellular redox environment was more efficient in condensing DNA. It was shown that s-PDMAEMA achieved higher gene transfection levels relative to their linear precursors and s-PDMAEMA200 with longer and more arms exhibited superior transfection efficiencies and lower cytotoxicity compared to PEI25K. The buffer capacities were examined by acid–base titration; the pH-dependent morphological evolution and enzyme stability of PDMAEMA/DNA complexes were investigated by atomic force microscopy (AFM) and time-resolved fluorescence spectroscopy, respectively. The results indicated that the star polymers exhibited a stronger buffering ability than their linear precursors due to the increased inner osmotic pressure. By decreasing the pH from 7.4 to 5.0, the linear PDMEMA/DNA complexes became more compact; in contrast, s-PDMAEMA200/DNA complex adopted a loose morphology due to the steric barrier of inter-arms and outward extension of positively charged arms. Analysis of the fluorescence life times of free and intercalated ethidium bromide unveiled more effective protection of DNA afforded by s-PDMAEMA. The effect of medium pH on the star PDMAEMA system was smaller owing to the ability of densely tertiary amino groups along multiple arms to absorb more protons, which was favorable for endosomolytic escape of complexes.

Keywords
Star polymer; N,N-dimethyl-ethylamine methacrylate; Redox-cleavable; Gene delivery; Atomic transfer radical polymerization
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Redox-cleavable star cationic PDMAEMA by arm-first approach of ATRP as a nonviral vector for gene delivery
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 3, January 2010, Pages 559–569
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us