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Nanoporous gold film encapsulating cytochrome c for the fabrication of a H2O2 biosensor

Paper ID Volume ID Publish Year Pages File Format Full-Text
8528 596 2009 6 PDF Available
Title
Nanoporous gold film encapsulating cytochrome c for the fabrication of a H2O2 biosensor
Abstract

A layer-by-layer route to prepare nanoporous Au film materials on transparent ITO substrates is reported by alternatively assembling Au and Ag nanoparticles through 1,5-pentanedithiol as a cross-linker, followed by that Ag nanoparticles are dissolved at room temperature in HAuCl4 solution. Electron transfer of cytochrome c (cyt. c) ā€“ an excellent model for investigation of biomolecules, is greatly facilitated at the nanoporous Au film with electron transfer rate constant (ks) of 3.9Ā sāˆ’1. Meanwhile, cyt. c adsorped onto the nanoporous Au film still maintain its enzymatic activity toward H2O2. On the basis of these experimental results, the cyt. c-nanoporous Au film is exploited to an amperometric biosensor for H2O2 with high selectivity, broad linear range, low detection limit, and long stability.

Keywords
Nanoporous Au film; Direct electron transfer; Biosensor; Cytochrome c; Hydrogen peroxide (H2O2)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 18, June 2009, Pages 3183ā€“3188
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us