fulltext.study @t Gmail

The encapsulation of DNA molecules within biomimetic lipid nanocapsules

Paper ID Volume ID Publish Year Pages File Format Full-Text
8530 596 2009 8 PDF Available
Title
The encapsulation of DNA molecules within biomimetic lipid nanocapsules
Abstract

Most of DNA synthetic complexes result from the self-assembly of DNA molecules with cationic lipids or polymers in an aqueous controlled medium. However, injection of such self-assembled complexes in medium like blood that differ from that of their formulation leads to strong instability. Therefore, DNA vectors that have physico-chemical properties and structural organisation that will not be sensitive to a completely different medium in terms of ionic and protein composition are actively sought. To this end, the goal here was to discover and optimize a nanostructured system where DNA molecules would be encapsulated in nanocapsules consisting in an oily core and a shell covered by PEG stretches obtained through a nanoemulsion process in the absence of organic solvent. This encapsulation form of DNA molecules would prevent interactions with external hostile biological fluid. The results show the entrapment of lipoplexes into lipid nanocapsules, leading to the formation of neutral 110 nm-DNA nanocapsules. They were weakly removed by the immune system, displaying an increased blood half-life, and improved carcinoma cell transfection, in comparison to the parent lipoplexes. Our results demonstrate that the fabrication of nanocapsules encapsulating hydrophilic DNA in an oily core that meet criteria for blood injection is possible.

Keywords
DNA; Liposome; Nanoparticle; Stealthiness; Poly(ethylene glycol); Blood
First Page Preview
The encapsulation of DNA molecules within biomimetic lipid nanocapsules
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 18, June 2009, Pages 3197–3204
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering