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In situ thermal gelling polypeptide for chondrocytes 3D culture

Paper ID Volume ID Publish Year Pages File Format Full-Text
8545 597 2010 7 PDF Available
Title
In situ thermal gelling polypeptide for chondrocytes 3D culture
Abstract

In the search for a cell-instructive or cell-interactive artificial extracellular matrix, synthetic hydrogels have been extensively investigated to apply three-dimensional (3D) cell culture and tissue engineering. Here, we are reporting a reverse thermal gelling l/dl-polyalanine block copolymer aqueous solution for chondrocyte 3D culture. The polymer aqueous solution undergoes sol-to-gel transition as the temperature increases, thus forming a 3D cell encapsulating scaffold in situ at 37 °C. In particular, the fraction of the β-sheet structure of the polyalanine dictated the population and thickness of fibrous nanostructure of the hydrogel, which in turn affected the proliferation and protein expression of the encapsulated chondrocytes. As an injectable tissue engineering system of chondrocytes, very promising results were confirmed for nude mice, using the current polypeptide aqueous solution. This paper not only provides important clues in designing an artificial extracellular matrix but also proves the significance of thermal gelling polypeptide as a minimally-invasive tissue engineering scaffold.

Keywords
Secondary structure; Peptide; Thermally responsive material; Hydrogel; Chondrocyte; Cell culture
First Page Preview
In situ thermal gelling polypeptide for chondrocytes 3D culture
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 35, December 2010, Pages 9266–9272
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering