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Transient in vitro epigenetic reprogramming of skin fibroblasts into multipotent cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
8565 598 2010 9 PDF Available
Title
Transient in vitro epigenetic reprogramming of skin fibroblasts into multipotent cells
Abstract

Multipotent stem cells have the potential to establish a new field of promising regenerative medicine to treat tissue damage, genetic disorders, and degenerative diseases. However, limited resource of stem cells has turned to be an evitable obstacle in clinical applications. We utilized a simple in vitro epigenetic reprogramming approach to convert skin fibroblasts into multipotent cells. After transient reprogramming, stem cell markers, including Oct4 and Nanog, became activated in the treated cells. The reprogrammed cells were multipotent as demonstrated by their ability to differentiate into a variety of cells and to form teratomas. Genomic imprinting of insulin-like growth factor II (Igf2) and H19 was not affected by this short period of cell reprogramming. This study may provide an alternative strategy to efficiently generate patient-specific stem cells for basic and clinical research, solving major hurdles of virally-induced pluripotent stem (iPS) cells that entail the potential risks of mutation, gene instability, and malignancy.

Keywords
Induced multipotent stem cells; iMS cells; Epigenetic reprogramming; Multipotency; Fish oocyte extracts; Differentiation
First Page Preview
Transient in vitro epigenetic reprogramming of skin fibroblasts into multipotent cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 10, April 2010, Pages 2779–2787
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering