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A versatile pH sensitive chondroitin sulfate–PEG tissue adhesive and hydrogel

Paper ID Volume ID Publish Year Pages File Format Full-Text
8566 598 2010 10 PDF Available
Title
A versatile pH sensitive chondroitin sulfate–PEG tissue adhesive and hydrogel
Abstract

We developed a chondroitin sulfate–polyethylene glycol (CS–PEG) adhesive hydrogel with numerous potential biomedical applications. The carboxyl groups on chondroitin sulfate (CS) chains were functionalized with N-hydroxysuccinimide (NHS) to yield chondroitin sulfate succinimidyl succinate (CS–NHS). Following purification, the CS–NHS molecule can react with primary amines to form amide bonds. Hence, using six arm polyethylene glycol amine PEG–(NH2)6 as a crosslinker we formed a hydrogel which was covalently bound to proteins in tissue via amide bonds. By varying the initial pH of the precursor solutions, the hydrogel stiffness, swelling properties, and kinetics of gelation could be controlled. The sealing/adhesive strength could also be modified by varying the damping and storage modulus properties of the material. The adhesive strength of the material with cartilage tissue was shown to be ten times higher than that of fibrin glue. Cells encapsulated or in direct contact with the material remained viable and metabolically active. Furthermore, CS–PEG material produced minimal inflammatory response when implanted subcutaneously in a rat model and enzymatic degradation was demonstrated in vitro. This work establishes an adhesive hydrogel derived from biological and synthetic components with potential application in wound healing and regenerative medicine.

Keywords
Bioactivity; Chondroitin sulfate; DMA (dynamic mechanical analysis); Hydrogel; Polyethylene oxide; Tissue adhesive
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A versatile pH sensitive chondroitin sulfate–PEG tissue adhesive and hydrogel
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 10, April 2010, Pages 2788–2797
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us