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Characterization of membrane materials and membrane coatings for bioreactor units of bioartificial kidneys

Paper ID Volume ID Publish Year Pages File Format Full-Text
8585 599 2011 12 PDF Available
Title
Characterization of membrane materials and membrane coatings for bioreactor units of bioartificial kidneys
Abstract

The bioreactor unit of bioartificial kidneys contains porous membranes seeded with renal cells. For clinical applications, it is mandatory that human primary renal proximal tubule cells (HPTCs) form differentiated epithelia on the membranes. Here, we show that HPTCs do not grow and survive on a variety of polymeric membrane materials. This applies also to membranes consisting of polysulfone/polyvinylpyrrolidone (PSF/PVP), which have been used in the bioreactor unit of bioartificial kidneys after coating with an extracellular matrix (ECM). Our data reveal that coating with just an ECM does not sufficiently improve HPTC performance on non-HPTC-compatible membrane materials. On the other hand, we have characterized the effects of a variety of surface treatments and coatings, and found that double coating with 3,4-dihydroxy-l-phenylalanine and an ECM markedly improves HPTC performance and results in the formation of differentiated epithelia on PSF/PVP membranes. We have also synthesized alternative membrane materials, and characterized membranes consisting of polysulfone and Fullcure™. We found that these membranes sustain proper HPTC performance without the need for surface treatments or coatings. Together, our data reveal that the materials that have been previously applied in bioartificial kidneys are not suitable for applications with HPTCs. This study elucidates the types of membrane materials and coatings that are favorable for the bioreactor unit of bioartificial kidneys.

Keywords
Biocompatibility; Bioreactor; Epithelial cell; Membrane; Polysulphone; Surface treatment
First Page Preview
Characterization of membrane materials and membrane coatings for bioreactor units of bioartificial kidneys
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 6, February 2011, Pages 1465–1476
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering