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In vitro generation of an osteochondral interface from mesenchymal stem cell–collagen microspheres

Paper ID Volume ID Publish Year Pages File Format Full-Text
8591 599 2011 10 PDF Available
Title
In vitro generation of an osteochondral interface from mesenchymal stem cell–collagen microspheres
Abstract

Creating biological interfaces between mechanically dissimilar tissues is a key challenge in complex tissue engineering. An osteochondral interface is essential in preventing mechanical failure and maintaining normal function of cartilage. Despite tremendous efforts in developing osteochondral plugs, formation of the osteochondral interface with proper zonal organization has not yet been reported. Here, we present a mesenchymal stem cell–collagen microsphere-based approach for complex tissue engineering and demonstrate in vitro formation of a stem cell-derived osteochondral interface with calcified cartilage interface separating a non-calcified cartilage layer and an underlying bone layer. Cells at the interface region are hypertrophic chondrocytes while the extracellular matrix in this region contains collagen type II and X, calcium deposits and vertically running fibers. The simultaneous presence of appropriate medium and configuration during co-culture is necessary for the interface formation.

Keywords
Complex tissue engineering; Microencapsulation; Mesenchymal stem cells; Collagen microspheres; Osteochondral interface; Calcified cartilage
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In vitro generation of an osteochondral interface from mesenchymal stem cell–collagen microspheres
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 6, February 2011, Pages 1526–1535
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us