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Co-electrospun dual scaffolding system with potential for muscle–tendon junction tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
8594 599 2011 11 PDF Available
Title
Co-electrospun dual scaffolding system with potential for muscle–tendon junction tissue engineering
Abstract

Tissue engineering has had successes developing single tissue types, but there is a need for methods that will allow development of composite tissues. For instance, muscle–tendon junctions (MTJ) require a seamless interface to allow force transfer from muscle to tendon. One challenge in engineering MTJs is designing a continuous scaffold suitable for both tissue types. We aimed to create a dual scaffold that exhibits regional mechanical property differences that mimic the trends seen in native MTJ. Poly(ε-caprolactone)/collagen and poly(l-lactide)/collagen were co-electrospun onto opposite ends of a mandrel to create a scaffold with 3 regions. Scaffolds were characterized with scanning electron microscopy, tensile testing (uniaxial, cyclic, and video strain), for cytocompatibility using MTS, and seeded with C2C12 myoblasts and NIH3T3 fibroblasts. Native porcine diaphragm MTJs were also analyzed with video strain for comparison. Integrated scaffolds were created with fiber diameters from 452–549 nm. Scaffolds exhibited regional variations in mechanical properties with moduli from 4.490–27.62 MPa and generally withstood cyclic testing, although with hysteresis. Video analysis showed scaffold strain profiles exhibited similar trends to native MTJ. The scaffolds were cytocompatible and accommodated cell attachment and myotube formation. The properties engineered into these scaffolds make them attractive candidates for tissue engineering of MTJs.

Keywords
Electrospinning; Mechanical properties; Muscle; Tendon; Strain profile; Composite tissue engineering
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Co-electrospun dual scaffolding system with potential for muscle–tendon junction tissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 6, February 2011, Pages 1549–1559
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us