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Controlled insulin release from glucose-sensitive self-assembled multilayer films based on 21-arm star polymer

Paper ID Volume ID Publish Year Pages File Format Full-Text
8615 599 2011 8 PDF Available
Title
Controlled insulin release from glucose-sensitive self-assembled multilayer films based on 21-arm star polymer
Abstract

A glucose-sensitive multilayer film was fabricated by the layer-by-layer (LbL) assembly method with positively charged 21-arm poly[2-(dimethylamino)ethyl methacrylate] (star PDMAEMA) and negatively charged insulin and glucose oxidase (GOD) in the form of {(Star PDMAEMA/Insulin)4 + (Star PDMAEMA/GOD)4 + Star PDMAEMA}. The multilayer film shows an on-off regulation of insulin release in response to stepwise glucose challenge in vitro. It is found that the unique structure of star PDMAEMA and interdiffusion of charged insulin are the main factors to control the on-off status of the film. Reversible surface morphology transitions of the multilayer film were also observed, revealing a phase separation and large-scale reorganization process. Furthermore, the multilayer film could continuously release enough insulin in vivo after being subcutaneously implanted in streptocozotin-induced diabetic rats and reduce the blood glucose level for at least two weeks. It is indicated that such system may have substantial potential as a glucose-sensitive carrier for insulin due to its distinct mechanism.

Keywords
Controlled release; Insulin; Multilayer films; Layer-by-layer assembly; 21-Arm star polymer; Animal experiments
First Page Preview
Controlled insulin release from glucose-sensitive self-assembled multilayer films based on 21-arm star polymer
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 32, Issue 6, February 2011, Pages 1759–1766
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering