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Differential effects of designed scaffold permeability on chondrogenesis by chondrocytes and bone marrow stromal cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
8628 600 2010 9 PDF Available
Title
Differential effects of designed scaffold permeability on chondrogenesis by chondrocytes and bone marrow stromal cells
Abstract

It has been widely postulated that scaffold permeability has a significant influence on chondrogenesis. However, since permeability has not been rigorously controlled in previous studies, there is no definitive conclusion as to how permeability affects cartilage regeneration by primary chondrocytes or progenitor cells. Here we explored the in vitro effects of scaffold permeability on matrix production and cellular differentiation of chondrocytes and bone marrow stromal cells (BMSCs) using precisely designed poly(ɛ-caprolactone) (PCL) scaffolds in which the high permeability design was 5.25 times more permeable than the lowest permeability design. We found that scaffold permeability affects the chondrogenic performance of chondrocytes and bone marrow stromal cells in opposite ways. Decreased scaffold permeability results in an increase in cartilaginous matrix production by chondrocytes, promoting increases in aggrecan content and collagen 2: collagen 1 gene expression ratios. On the other hand, increased scaffold permeability is more favorable for differentiation of BMSCs down a chondrogenic lineage in this model. The ability to direct cell differentiation and chondrogenesis through a physical design parameter, such as permeability, establishes the capability to incorporate chondrogenic potential into a material design.

Keywords
Scaffold; Polycaprolactone; Cartilage tissue engineering; Chondrocyte; Bone marrow stromal cell
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Differential effects of designed scaffold permeability on chondrogenesis by chondrocytes and bone marrow stromal cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 2, January 2010, Pages 279–287
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us