fulltext.study @t Gmail

In vitro generation of mechanically functional cartilage grafts based on adult human stem cells and 3D-woven poly(ɛ-caprolactone) scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
8673 602 2010 8 PDF Available
Title
In vitro generation of mechanically functional cartilage grafts based on adult human stem cells and 3D-woven poly(ɛ-caprolactone) scaffolds
Abstract

Three-dimensionally woven poly(ɛ-caprolactone) (PCL) scaffolds were combined with adult human mesenchymal stem cells (hMSC) to engineer mechanically functional cartilage constructs in vitro. The specific objectives were to: (i) produce PCL scaffolds with cartilage-like mechanical properties, (ii) demonstrate that hMSCs formed cartilage after 21 days of culture on PCL scaffolds, and (iii) study effects of scaffold structure (loosely vs. tightly woven), culture vessel (static dish vs. oscillating bioreactor), and medium composition (chondrogenic additives with or without serum). Aggregate moduli of 21-day constructs approached normal articular cartilage for tightly woven PCL cultured in bioreactors, were lower for tightly woven PCL cultured statically, and lowest for loosely woven PCL cultured statically (p < 0.05). Construct DNA, total collagen, and glycosaminoglycans (GAG) increased in a manner dependent on time, culture vessel, and medium composition. Chondrogenesis was verified histologically by rounded cells within a hyaline-like matrix that immunostained for collagen type II but not type I. Bioreactors yielded constructs with higher collagen content (p < 0.05) and more homogenous matrix than static controls. Chondrogenic additives yielded constructs with higher GAG (p < 0.05) and earlier expression of collagen II mRNA if serum was not present in medium. These results show feasibility of functional cartilage tissue engineering from hMSC and 3D-woven PCL scaffolds.

Keywords
Cartilage repair; Stem cell; Bioreactor; Biomechanics; Growth factors
First Page Preview
In vitro generation of mechanically functional cartilage grafts based on adult human stem cells and 3D-woven poly(ɛ-caprolactone) scaffolds
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 8, March 2010, Pages 2193–2200
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us