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Control of cell attachment through polyDNA hybridization

Paper ID Volume ID Publish Year Pages File Format Full-Text
8677 602 2010 7 PDF Available
Title
Control of cell attachment through polyDNA hybridization
Abstract

Cell–cell interactions play vital roles in embryo development and in homeostasis maintenance. Such interactions must be stringently controlled for cell-based tissue engineering and regenerative medicine therapies, and methods for studying and controlling cell–cell interactions are being developed using both biomedical and engineering approaches. In this study, we prepared amphiphilic PEG-lipid polymers that were attached to polyDNA with specific sequences. Incubation of cells with the polyDNA–PEG-lipid conjugate transferred some of the polyDNA to the cells' surfaces. Similarly, polyDNA–PEG-lipid conjugate using polyDNA with a complementary sequence was introduced to the surfaces of other cells or to a substrate surface. Cell–cell or cell–substrate attachments were subsequently mediated via hybridization between the two complementary polyDNAs and monitored using fluorescence microscopy.

Keywords
Cell–cell attachment; Poly(ethylene glycol)-lipid (PEG-lipid); DNA hybridization; Surface modification
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Control of cell attachment through polyDNA hybridization
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 8, March 2010, Pages 2229–2235
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us