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Immobilization of the soluble domain of human complement receptor 1 on agarose-encapsulated islets for the prevention of complement activation

Paper ID Volume ID Publish Year Pages File Format Full-Text
8729 604 2010 7 PDF Available
Title
Immobilization of the soluble domain of human complement receptor 1 on agarose-encapsulated islets for the prevention of complement activation
Abstract

The transplantation of islets of Langerhans has been successfully applied to the treatment of insulin-dependent diabetes. However, a shortage of human donors is the hardest obstacle to overcome. We aimed to develop a bioartificial pancreas that can realize xeno-islet transplantation. The islets were encapsulated in agarose microbeads carrying the soluble domain of human complement receptor 1 (sCR1), which is an effective inhibitor of the classical and alternative complement activation pathways. When naked rat islets were cultured in rabbit serum, large amounts of insulin leaked from the damaged islets over the course of a few days incubation, but no damaged cells were observed among islets in sCR1-agarose microbeads cultured in rabbit serum for 4 days. Although low levels of insulin were detected in the rabbit serum, the insulin did not leak from damaged β-cells, it was physiological insulin secreted by the β-cells.

Keywords
Islets of Langerhans; Bioartificial pancreas; Agarose; Soluble complement receptor 1 (sCR1); Microencapsulation; Immuno-isolation
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Immobilization of the soluble domain of human complement receptor 1 on agarose-encapsulated islets for the prevention of complement activation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 34, December 2010, Pages 8847–8853
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us