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Osteoinductive silk–silica composite biomaterials for bone regeneration

Paper ID Volume ID Publish Year Pages File Format Full-Text
8735 604 2010 9 PDF Available
Title
Osteoinductive silk–silica composite biomaterials for bone regeneration
Abstract

Osteoinductive and biodegradable composite biomaterials for bone regeneration were prepared by combining silk fibroin with silica particles. The influence of these composite systems on osteogenesis was evaluated with human mesenchymal stem cells (hMSCs) subjected to osteogenic differentiation. hMSCs adhered, proliferated, and differentiated towards osteogenic lineages on silk/silica films. The addition of the silica to the silk films influenced gene expression leading to upregulation of bone sialoprotein (BSP) and collagen type 1 (Col 1) osteogenic markers. Evidence for early bone formation in the form of collagen fibers and apatite nodules was obtained on the silk/silica films. Collagen fibers were closely associated with apatite deposits and overall collagen content was higher for the silica containing samples. Also, smaller sized silica particles (24 nm–2 μm) with large surface area facilitated silica biodegradation in vitro through particle dissolution, leading to ∼5-fold decrease in silica content over 10 weeks. These results indicate the suitability of silk/silica composite system towards bone regeneration, where degradation/remodeling rates of the organic and inorganic components can be controlled.

Keywords
Silk; Silica; Osteogenesis; Nanoparticles; Composite; Stem cells
First Page Preview
Osteoinductive silk–silica composite biomaterials for bone regeneration
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 34, December 2010, Pages 8902–8910
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering