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Existence of a typical threshold in the response of human mesenchymal stem cells to a peak and valley topography

Paper ID Volume ID Publish Year Pages File Format Full-Text
874 68 2011 10 PDF Available
Title
Existence of a typical threshold in the response of human mesenchymal stem cells to a peak and valley topography
Abstract

Our objective in this study was to determine whether a threshold in sensitivity of human mesenchymal stem cells (hMSC) to isotropic roughness exists. Using electrical discharge machining a very wide range of roughnesses (1.2 μm < Ra < 21 μm) with a perfectly isotropic, fractal and self-affine topography can be produced on titanium, with a range of roughness overlapping the hMSC length dimensions. The curve of the number of adherent hMSC after 2 days culture as a function of roughness showed a U-shape with a minimum number of attached cells for a roughness amplitude Ra = 4.5 μm and a distance between surface features (width of peaks and valleys) Sm = 110 μm. The maximum cell number was observed at the lowest and highest roughnesses. Due to this very wide range of roughness it was possible to demonstrate that the response of hMSC to roughness varies with the dimensions of the surface features relative to the cell size. Above or below their own size hMSC essentially adhere to the nano and submicron features. When the surface displays features about the same size as hMSC the curvature of these surface features will decrease the number of attached cells by a factor of two. A modelling approach is proposed to help the interpretation of these results. It is hypothesized that this minimal adhesion is a consequence of an unfavourable stress imposed on the cell cytoskeleton.

Keywords
Roughness; Titanium; Cell adhesion; hMSCs; Modelling
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Existence of a typical threshold in the response of human mesenchymal stem cells to a peak and valley topography
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 9, September 2011, Pages 3302–3311
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us