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A high throughput micro-array system of polymer surfaces for the manipulation of primary pancreatic islet cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
8745 604 2010 7 PDF Available
Title
A high throughput micro-array system of polymer surfaces for the manipulation of primary pancreatic islet cells
Abstract

We developed a high throughput micro-arrayed polymer system for the study of polymer surfaces for islet cell culture. A micro-arrayed library with 496 different polymers was synthesized and used to examine attachment and insulin expression of islet cells. While most polymers were not supportive, several related polymers were identified as suitable (“hit’s”). The “hit” arrays composed of “hit” polymers with 36 replicates were fabricated to confirm their capacities to support the attachment of islet cells, and these capacities were further validated in large surfaces. Notably, the attachment of islet cells on these synthetic polymeric films has been found to be as supportive as 804G supernatant coated tissue culture polystyrene dishes, one of the most extensively used substrates for the islet cell attachment. Interestingly, the polymeric surfaces optimal for a different cell type, hES derived cells, were distinct, highlighting the utility of these approaches for identifying cell type specific surfaces.

Keywords
Pancreatic islet cells; Cell type specific adhesion; Polymer array; High throughput screening
First Page Preview
A high throughput micro-array system of polymer surfaces for the manipulation of primary pancreatic islet cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 34, December 2010, Pages 8989–8995
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering