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The MRI assessment of intraurethrally – delivered muscle precursor cells using anionic magnetic nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
8775 605 2009 9 PDF Available
Title
The MRI assessment of intraurethrally – delivered muscle precursor cells using anionic magnetic nanoparticles
Abstract

Autografting of cultured myogenic precursor cells (MPC) is a therapeutic strategy for muscle disorders, including striated urethral sphincter insufficiency. Implantation of myofibers with their satellite cells into the urethra is a recently described method of MPC transfer aimed at generating a new sphincter in incontinent patients. In this study, we magnetically labeled muscle implants with dextran-free anionic iron oxide nanoparticles (AMNP). The aim was to evaluate the biocompatibility of the labeling procedure and its utility for non-invasive MRI follow-up of cell therapy in a female pig model. After adsorption of AMNP to the implant surface, various cell types, including MPC, were magnetically labeled within the implants. Magnetic labeling did not affect cell proliferation or differentiation. Autograft detection in vivo by 0.3-T MRI was possible for up to 1 month. Ex vivo, Perl's, anti-desmin and anti-myosin heavy chain staining confirmed the co-localization of AMNP and regenerated myofibers. AMNP labeling was thus useful for locating myofiber implant autografts in vivo and for ex vivo monitoring of the biology of this cell transfer method.

Keywords
Iron oxide nanoparticles; MRI; Urethral sphincter; Cell labeling; Tissue labeling; Muscle cell therapy
First Page Preview
The MRI assessment of intraurethrally – delivered muscle precursor cells using anionic magnetic nanoparticles
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 36, December 2009, Pages 6920–6928
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering