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The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system

Paper ID Volume ID Publish Year Pages File Format Full-Text
8790 606 2009 7 PDF Available
Title
The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system
Abstract

3D-microfluidic cell culture systems (3D-μFCCSs) support hepatocyte functions in vitro which can be further enhanced by controlled presentation of 100–200 pg/ml TGF-β1, thus mimicking the roles of supporting cells in co-cultures. Controlled presentation of TGF-β1 is achieved by either direct perfusion or in situ controlled release from gelatin microspheres immobilized in the 3D-μFCCS. Primary hepatocytes cultured for 7 days with the in situ controlled released TGF-β1 exhibited up to four-fold higher albumin secretion and two-fold higher phase I/II enzymatic activities, significantly improving the sensitivity of hepatocytes to acetaminophen-mediated hepatotoxicity, compared to hepatocytes cultured with directly perfused TGF-β1 or without TGF-β1. The controlled presentation of TGF-β1 enhanced hepatocyte functions in microfluidic systems without the complications of co-cultures, allowing for simplifications in drug testing and other hepatocyte-based applications.

Keywords
Transforming growth factor; Hepatocyte co-culture; 3D cell constructs; Microfluidics; Controlled release; Soluble microenvironment
First Page Preview
The controlled presentation of TGF-β1 to hepatocytes in a 3D-microfluidic cell culture system
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issues 23–24, August 2009, Pages 3847–3853
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering