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Fabrication of hASCs-laden structures using extrusion-based cell printing supplemented with an electric field

Paper ID Volume ID Publish Year Pages File Format Full-Text
88 6 2016 11 PDF Available
Title
Fabrication of hASCs-laden structures using extrusion-based cell printing supplemented with an electric field
Abstract

In this study, we proposed a hybrid cell-printing technique that combines a conventional extrusion-based cell-printing process with an electrohydrodynamic jet. The electric field stabilized the extruded struts of cell-embedding-hydrogel and reduced the damage to dispensed cells caused by the high wall shear stress in the dispensing nozzle. The new cell-printing process was optimized in terms of various processing parameters, applied electric field strength, nozzle movement speed, and distance between the nozzle tip and working stage. Using the optimal cell-embedding hydrogel composition (1 × 106 cells mL−1 in 4 wt% alginate) and cell-printing process parameters (applied voltage, 1 kV; nozzle movement speed, 12 mm s−1; distance, 0.7 mm; current, 10.67 ± 1.1 nA), we achieved rapid and stable fabrication of a cell-laden structure without loss of cell viability or proliferation, the values of which were similar to those of the process without an electric field. Furthermore, by applying the same pneumatic pressure to fabricate cell-laden structures, considerably higher volume flow rate and cell viability at the same volume flow rate were achieved by the modified process compared with conventional extrusion-based cell-printing processes. To assess the feasibility of the method, the hydrogel containing human adipose stem cells (hASCs) and alginate (4 wt%) was fabricated into a cell-laden porous structure in a layer-by-layer manner. The cell-laden structure exhibited reasonable initial hASC viability (87%), which was similar to that prior to processing of the cell-embedding-hydrogel.Statement of SignificanceThe extrusion-based cell-printing process has shortcomings, such as unstable flow and potential loss of cell viability. The unsteady flow can occur due to the high cell concentration, viscosity, and surface tension of bioinks. Also, cell viability post extrusion can be significantly reduced by damage of the cells due to the high wall shear stress in the extrusion nozzle. To overcome these limitations, we suggested an innovative cell-printing process that combines a conventional extrusion-based cellprinting process with an electric field. The electric field in the cell-printing process stabilized the extruded struts of bioink and dramatically reduced the damage to dispensed cells caused by the high wall shear stress in the dispensing nozzle.

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Keywords
Cell printing; Electrohydrodynamic jet; Human adipose stem cells; Cell-laden structure
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Fabrication of hASCs-laden structures using extrusion-based cell printing supplemented with an electric field
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 38, 1 July 2016, Pages 33–43
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us