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Masking and triggered unmasking of targeting ligands on nanocarriers to improve drug delivery to brain tumors

Paper ID Volume ID Publish Year Pages File Format Full-Text
8804 606 2009 10 PDF Available
Title
Masking and triggered unmasking of targeting ligands on nanocarriers to improve drug delivery to brain tumors
Abstract

Long-circulating nanocarriers have been extensively studied to deliver chemotherapeutics; however, the inclusion of targeting agents compromises circulation times thereby offsetting the benefits of active targeting. Here, we formulated cysteine-cleavable phospholipid–polyethylene glycol (PEG) to ‘mask’ nanocarrier bound targeting ligands from RES clearance and prolong circulation times of liposomes to allow passive targeting to tumors. This detachable polymer coating can be removed after nanocarrier extravasation to tumor is achieved to expose targeting ligands and promote active targeting to tumor cells. In vivo studies on folate receptor-targeted liposomes demonstrated our ability to prolong circulation in the bloodstream using this system thereby verifying the ‘masking’ capacity of cleavable phospholipid–PEG5000. Controlled modulation of uptake and cytotoxicity of targeted nanocarriers using cleavable phospholipid–PEG was demonstrated through in vitro studies. Finally, studies analyzing uptake by tumor cells in vivo confirmed enhanced intracellular delivery when tumor-inoculated animals received targeted liposomes containing cleavable phospholipid–PEG5000 followed by a cysteine infusion to expose folate after liposomes had extravasated to tumor. These results indicate that cleavable phospholipid–PEG can be used in nanocarrier formulations for controlled exposure of targeting ligands to ensure that circulation times remain uncompromised by the inclusion of targeting agents while enabling active targeting to tumors after removal of the polymer coating.

Keywords
Liposome; Folate; Glioma; Targeting; Cleavable PEG; Nanocarrier
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Masking and triggered unmasking of targeting ligands on nanocarriers to improve drug delivery to brain tumors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issues 23–24, August 2009, Pages 3986–3995
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us