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The effect of triethylene glycol dimethacrylate on p53-dependent G2 arrest in human gingival fibroblasts

Paper ID Volume ID Publish Year Pages File Format Full-Text
8836 608 2010 9 PDF Available
Title
The effect of triethylene glycol dimethacrylate on p53-dependent G2 arrest in human gingival fibroblasts
Abstract

Dental resin composites have been reported to exert adverse effects on cells of the oral cavity. In this study, we demonstrate that a non-cytotoxic concentration of the resin co-monomer triethylene glycol dimethacrylate (TEGDMA) results in the reduction of the proliferation rate of human gingival fibroblasts (HGFs), by delaying them at the G2 phase of the cell cycle, and in the sustained production of reactive oxygen species. These phenomena are accompanied by an early transient de-phosphorylation of ERK1/2 and JNKs and a late activation of the p53-p21WAF1-pRb molecular pathway. By using siRNA-mediated knocking down of the human p53 gene, we present evidence that the onco-suppressive protein p53 controls the TEGDMA-activated G2 checkpoint in HGFs and prevents their entry into mitosis, possibly in order to protect them from the detrimental genotoxic effects of the compound.

Keywords
TEGDMA; Proliferation; Cell cycle; MAPKs; DCFH-DA; p53 siRNA
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The effect of triethylene glycol dimethacrylate on p53-dependent G2 arrest in human gingival fibroblasts
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 33, November 2010, Pages 8530–8538
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us