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Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous Scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
8849 608 2010 8 PDF Available
Title
Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous Scaffolds
Abstract

Visualizing cells in three-dimensional (3D) scaffolds has been one of the major challenges in tissue engineering. Most current imaging modalities either suffer from poor penetration depth or require exogenous contrast agents. Here, we demonstrate photoacoustic microscopy (PAM) of the spatial distribution and temporal proliferation of cells inside three-dimensional porous scaffolds with thicknesses over 1 mm. Specifically, we evaluated the effects of seeding and culture methods on the spatial distribution of melanoma cells. Spatial distribution of the cells in the scaffold was well-resolved in PAM images. Moreover, the number of cells in the scaffold was quantitatively measured from the as-obtained volumetric information. The cell proliferation profile obtained from PAM correlated well with what was obtained using the traditional 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.

Keywords
Inverse opal scaffolds; Photoacoustic microscopy; Melanoma; Tissue engineering; Biomedical imaging
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Chronic label-free volumetric photoacoustic microscopy of melanoma cells in three-dimensional porous Scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 33, November 2010, Pages 8651–8658
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us