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Phenotype and functional analysis of human monocytes-derived dendritic cells loaded with a carbosilane dendrimer

Paper ID Volume ID Publish Year Pages File Format Full-Text
8860 608 2010 10 PDF Available
Title
Phenotype and functional analysis of human monocytes-derived dendritic cells loaded with a carbosilane dendrimer
Abstract

Dendritic cells (DCs) play a major role in development of cell-mediated immunotherapy due to their unique role in linking innate and adaptive immunities. In spite of improvement in this area, strategies employing ex vivo generated DCs have shown limited efficacy in clinical trials. Dendrimers have been proposed as new carriers for drug delivery in aim to ameliorate DCs antigen loading that is a pivotal point in DCs approaches. In this study, we have investigated the phenotypic and functional characteristics of human monocytes-derived dendritic cells after HIV-derived peptides uptake in vitro. We have found that iDCs and mDCs were able to capture efficiently water soluble carbosilane (CBS) dendrimer 2 G-NN16 and did not induce changes in maturation markers levels at the DCs surface. Therefore, CBS 2 G-NN16-loaded mDCs migrated as efficiently as unloaded DCs towards CCL19 or CCL21. Furthermore, DCs viability, activation of allogenic naïve CD4 + T cells by mDCs and secretion of cytokines were not significantly changed by 2 G-NN16 loading. Summing up, our data indicate that CBS 2 G-NN16 has no negative effects on the pivotal properties of DCs in vitro. It should therefore be feasible to further develop this antigen loading strategy for clinical use in immunotherapy against viral infections.

Keywords
Dendritic cells; Carbosilane dendrimer 2 G-NN16; Immunomodulation; Nanoparticle; PeptidemAbs, monoclonal antibody; ART, active antiretroviral therapy; CBS, carbosilane; CFSE, carboxyfluorescein succinimidyl ester; CCL19 or CCL21, C–C motif chemokine 19 or
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Phenotype and functional analysis of human monocytes-derived dendritic cells loaded with a carbosilane dendrimer
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 33, November 2010, Pages 8749–8758
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering