fulltext.study @t Gmail

Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147

Paper ID Volume ID Publish Year Pages File Format Full-Text
8864 608 2010 13 PDF Available
Title
Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147
Abstract

We present the preparation and isolation of different chemically modified BSA species with varying numbers of primary amino groups at the surface. Highly cationic albumin proteins with increased numbers of amino groups were achieved and complex formation with plasmid DNA was carefully investigated. We compare the transfection results, polyelectrolyte complexes morphologies with their impact on complex stabilities, cytotoxicities and DNA accessibility. This knowledge-driven approach led to the identification of the efficient non-viral DNA delivery agent cBSA-147, which showed high transfection efficacies and stability.

Keywords
Cationized serum albumin; Protein transfection agent; cBSA-147; Polyelectrolyte complex formation; Isothermal titration calorimetry
First Page Preview
Fine-tuning DNA/albumin polyelectrolyte interactions to produce the efficient transfection agent cBSA-147
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 33, November 2010, Pages 8789–8801
Authors
, , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us