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Perichondrium directed cartilage formation in silk fibroin and chitosan blend scaffolds for tracheal transplantation

Paper ID Volume ID Publish Year Pages File Format Full-Text
887 68 2011 10 PDF Available
Title
Perichondrium directed cartilage formation in silk fibroin and chitosan blend scaffolds for tracheal transplantation
Abstract

The purpose of this study was to investigate the potential of silk fibroin and chitosan blend (SFCS) biological scaffolds for the purpose of cartilage tissue engineering with applications in tracheal tissue reconstruction. The capability of these scaffolds as cell carrier systems for chondrocytes was determined in vitro and cartilage generation in vivo on engineered chondrocyte–scaffold constructs with and without a perichondrium wrapping was tested in an in vivo nude mouse model. SFCS scaffolds supported chondrocyte adhesion, proliferation, and differentiation, determined as features of the cells based on the spherical cell morphology, increased accumulation of glycosaminoglycans, and increased collagen type II deposition with time within the scaffold framework. Perichondrium wrapping significantly (P < 0.001) improved chondrogenesis within the cell–scaffold constructs in vivo. In vivo implantation for 6 weeks did not generate cartilage structures resembling native trachea, although cartilage-like structures were present. The mechanical properties of the regenerated tissue increased due to the deposition of chondrogenic matrix within the SFCS scaffold structural framework of the trachea. The support of chondrogenesis by the SFCS tubular scaffold construct resulted in a mechanically sound structure and thus is a step towards an engineered trachea that could potentially support the growth of an epithelial lining resulting in a tracheal transplant with properties resembling those of the fully functional native trachea.

Keywords
Biodegradable biological scaffold; Silk fibroin; Chitosan; Cartilage tissue engineering; Tracheal regeneration
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Perichondrium directed cartilage formation in silk fibroin and chitosan blend scaffolds for tracheal transplantation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 9, September 2011, Pages 3422–3431
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us