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Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2

Paper ID Volume ID Publish Year Pages File Format Full-Text
8879 609 2010 12 PDF Available
Title
Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2
Abstract

Ex vivo expansion of hematopoietic stem cells has been explored in the fields of stem cell biology, gene therapy and clinical transplantation. Recently, we demonstrated the existence of a circulating myogenic progenitor expressing the CD133 antigen. The relative inability of circulating CD133+ stem cells to reproduce themselves ex vivo imposes substantial limitations on their use for clinical applications in muscular dystrophies. Here we report that the use of cluster-assembled nanostructured titanium dioxide (ns-TiO2) substrates, in combination with cytokine enriched medium, enables high-level expansion of circulating CD133+ stem cells in vitro. Furthermore, we demonstrate that expanded circulating CD133+ stem cells retain their in vitro capacity to differentiate into myogenic cells. The exploitation of cluster-assembled ns-TiO2 substrates for the expansion of CD133+ stem cells in vitro could therefore make the clinical application of these stem cells for the treatment of muscle diseases practical.

Keywords
Stem cell; Titanium oxide; Nanotopography; Surface treatment; Cell culture; Muscular dystrophy
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Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 20, July 2010, Pages 5385–5396
Authors
, , , , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us