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Protein expression profiles in osteoblasts in response to differentially shaped hydroxyapatite nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
8905 610 2009 7 PDF Available
Title
Protein expression profiles in osteoblasts in response to differentially shaped hydroxyapatite nanoparticles
Abstract

The use of synthetic hydroxyapatite as bone substitute calls for the knowledge of the influence on adjacent cells. The aim of this study was to investigate the proteins with differential protein expression levels in the proteome of human osteoblast cell line incubated separately with various nano sized hydroxyapatite powders with different shapes and chemical compositions using iTRAQ-coupled 2D LC-MS/MS approach. In the present study, we investigated several intracellular signaling molecules involved in calcium regulation to analyze how osteoblast cells respond to dissimilar HA nanoparticles. It was found there was a significant decrease in cell population after adding the HA nanoparticles to the osteoblasts. Our results combining proteomics analysis and RT-PCR validation on targeted genes involved in calcium regulation confirmed the differences in the cellular response to dissimilar HA nanoparticles.

Keywords
Nanoparticles; Hydroxyapatite; Osteoblast; iTRAQ; Protein profile; LC-MS/MS analysis
First Page Preview
Protein expression profiles in osteoblasts in response to differentially shaped hydroxyapatite nanoparticles
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 29, October 2009, Pages 5385–5391
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering