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Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing

Paper ID Volume ID Publish Year Pages File Format Full-Text
8910 610 2009 6 PDF Available
Title
Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing
Abstract

The purpose of the present study is to develop a novel method for the fabrication of transferable micropatterned cell sheets for tissue engineering. To achieve this development, microcontact printing of fibronectin on commercially available temperature-responsive dishes was employed. Primary rat hepatocytes were seeded on the dish surfaces printed with fibronectin. Under serum-free conditions, hepatocytes were attached onto fibronectin domains selectively. Then, a second cell type of endothelial cells was seeded in the presence of serum. Double fluorescent staining revealed that endothelial cells successfully adhered to the intervals of hepatocyte domains. Finally, all the cells were harvested as a single contiguous micropatterned cell sheet upon temperature-reduction. With a cell sheet manipulator having a gelatin layer for the support of harvested cell sheets, harvested micropatterned cell sheets were transferred to new dish surfaces. This technique would be useful for the fabrication of thick tissue constructs having a complex microarchitecture.

Keywords
Micropatterning; Microcontact printing; Co-culture; Cell sheet; Maskless photolithography; Tissue engineering
First Page Preview
Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 29, October 2009, Pages 5427–5432
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering