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The binding of pullulan modified cholesteryl nanogels to Aβ oligomers and their suppression of cytotoxicity

Paper ID Volume ID Publish Year Pages File Format Full-Text
8926 610 2009 9 PDF Available
Title
The binding of pullulan modified cholesteryl nanogels to Aβ oligomers and their suppression of cytotoxicity
Abstract

Among various hydrogels able to form monodisperse and stable nanoparticles (20–30 nm) are those with pullulan-bearing cholesteryl moieties (CHP). These nanoparticles can interact with soluble proteins through hydrophobic bonding. The objectives of this study were to investigate whether CHP nanogels would interact with oligomeric forms of the 42 amino acid variant of β-amyloid (Aβ1–42) and if the formation of CHP-Aβ1–42 oligomer entities will reduce cytotoxicity of Aβ1–42 in primary cortical cells and microglial (N9) cells. By employing fluorescent CHP analogs with different charges we provide evidence that, (i) both neutral and positively charged CHP nanoparticles interact with Aβ1–42 monomers and oligomers, (ii) neutral CHP is non-toxic, but positively charged derivatives (CHPNH2) are toxic, particularly in primary cortical cultures, and (iii) binding of both monomeric and oligomeric Aβ1–42 to CHP significantly reduces Aβ1–42 toxicity in both the primary cortical and microglial cells. These results suggest that CHP nanogels could provide a valid complementary approach to antibody immunotherapy in neurological disorders characterized by the formation of soluble toxic aggregates, such as those in Alzheimer's disease (AD).

Keywords
Nanogel; β-Amyloid (Aβ); Alzheimer's disease (AD); Oligomers
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The binding of pullulan modified cholesteryl nanogels to Aβ oligomers and their suppression of cytotoxicity
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 29, October 2009, Pages 5583–5591
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us