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Synthesis, characterization, and in vivo targeted imaging of amine-functionalized rare-earth up-converting nanophosphors

Paper ID Volume ID Publish Year Pages File Format Full-Text
8927 610 2009 9 PDF Available
Title
Synthesis, characterization, and in vivo targeted imaging of amine-functionalized rare-earth up-converting nanophosphors
Abstract

Rare-earth up-converting nanophosphors (UCNPs) have great potential to become a new generation of biological luminescent labels, but their use has been limited by difficulties in obtaining water-soluble UCNPs bearing appropriate functional groups. To address this problem, we report herein a simple and efficient procedure for the preparation of amine-functionalized UCNPs by a modified hydrothermal microemulsion route assisted with 6-aminohexanoic acid. The amine content of the resultant UCNPs has been determined to be (9.5 ± 0.8) × 10−5 mol/g, which not only confers excellent dispersibility in aqueous solution, but also allows further conjugation with targeted molecules such as folic acid (FA) as a ligand. By means of the laser scanning up-conversion luminescence microscopy (LSUCLM) and the in vivo up-conversion luminescence (UCL) imaging under excitation at the CW infrared laser at 980 nm, FA-coupled UCNPs have been demonstrated to be effective in targeting folate-receptor overexpressing HeLa cells in vitro and HeLa tumor in vivo and ex vivo. These results indicated that our UCNPs could be used as whole-body targeted UCL imaging agents.

Keywords
Rare-earth nanophosphors; Up-conversion luminescence; Modified hydrothermal microemulsion synthesis; In vivo imaging; Amine-functionalized
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Synthesis, characterization, and in vivo targeted imaging of amine-functionalized rare-earth up-converting nanophosphors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 29, October 2009, Pages 5592–5600
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us