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The development of photochemically crosslinked native fibrinogen as a rapidly formed and mechanically strong surgical tissue sealant

Paper ID Volume ID Publish Year Pages File Format Full-Text
8970 611 2009 7 PDF Available
Title
The development of photochemically crosslinked native fibrinogen as a rapidly formed and mechanically strong surgical tissue sealant
Abstract

We recently reported the generation of a highly elastic, crosslinked protein biomaterial via a rapid photochemical process using visible light illumination. In light of these findings, we predicted that other unmodified, tyrosine-rich, self-associating proteins might also be susceptible to this covalent crosslinking method. Here we show that unmodified native fibrinogen can also be photochemically crosslinked into an elastic hydrogel biomaterial through the rapid formation of intermolecular dityrosine. Photochemically crosslinked fibrinogen forms tissue sealant bonds at least 5-fold stronger than commercial fibrin glue and is capable of producing maximum bond strength within 20 s. In vitro studies showed that components of the photochemical crosslinking reaction are non-toxic to cells. This material will find useful application in various surgical procedures where rapid curing for high strength tissue sealing is required.

Keywords
Fibrinogen; Tissue sealant; Photochemical crosslinking; Dityrosine; Ruthenium; Biomaterial
First Page Preview
The development of photochemically crosslinked native fibrinogen as a rapidly formed and mechanically strong surgical tissue sealant
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 30, Issue 11, April 2009, Pages 2059–2065
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering