fulltext.study @t Gmail

A rabbit anterior cornea replacement derived from acellular porcine cornea matrix, epithelial cells and keratocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
8986 612 2010 9 PDF Available
Title
A rabbit anterior cornea replacement derived from acellular porcine cornea matrix, epithelial cells and keratocytes
Abstract

The aim of this study was to construct a rabbit anterior cornea replacement with an acellular porcine cornea matrix (APCM) as a scaffold. The scaffold was prepared from fresh porcine corneas which were treated with 0.5% (wt./vol.) sodium dodecyl sulfate (SDS) solution and stirred for 24 h in a 4 °C refrigeration chamber. The complete removal of corneal cells was confirmed by H&E and DAPI staining. The stroma structure and mechanical properties were well preserved. The extracts had no cytotoxicity to rabbit corneal keratocytes, epithelial and endothelial cells as determined by MTT assay. Moreover, there was no sign that an immune reaction occurred in or around the transplanted disks within 6 months of animal implantation. To construct a rabbit anterior cornea replacement, keratocytes were injected into APCM and cultured for 7 days in a dynamic culturing system, followed by culturing corneal epithelial cells on the stroma construct surface for another 7 days. The phenotype of the construct was similar to normal rabbit corneas, with high expression of cytokeratin 3 in the epithelial cell layer and expression of vimentin in the stromal cells. These results suggested that the APCM developed by using SDS might be a suitable scaffold for cornea tissue engineering.

Keywords
Cornea; Decellularization; Scaffold; Sodium dodecyl sulfate; Tissue engineering
First Page Preview
A rabbit anterior cornea replacement derived from acellular porcine cornea matrix, epithelial cells and keratocytes
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 28, October 2010, Pages 7257–7265
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us