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Cytotoxicity and in vivo tissue compatibility of poly(amidoamine) with pendant aminobutyl group as a gene delivery vector

Paper ID Volume ID Publish Year Pages File Format Full-Text
9002 614 2010 10 PDF Available
Title
Cytotoxicity and in vivo tissue compatibility of poly(amidoamine) with pendant aminobutyl group as a gene delivery vector
Abstract

To design successful polymeric gene delivery vehicles with good biocompatibility and highly efficient gene transfer ability is one of the great scientific challenges in modern gene therapy. Poly(amidoamine) with pendant aminobutyl group (PAA-BA) has been proved to exhibit high transfection efficiency against bone marrow stromal cells (BMSCs) in vitro. In this work, based on previous research, PAA-BA's biocompatibility including in vitro cytotoxicity determined by effect on BMSCs' morphology, viability, membrane damage and apoptosis/necrosis, and in vivo tissue compatibility determined by muscular and hepatic tissue response were further investigated in comparison to branched polyethylenimine (PEI) 25 kDa. The results demonstrated that PAA-BA possess much better cytocompatibility than PEI, yielding slight cell morphological change, high cell viability and mild effect on cell membrane damage as well as inducing less apoptotic/necrotic cells at optimal N/P ratio. PAA-BA also exhibited better tissue compatibility, reflected by no or less inflammatory response in the site of muscle injection at the same (0.03% w/v) or higher concentration (0.1% w/v) and no hepatic tissue morphological change with normal hepatocytes. We concluded that PAA-BA was promising and safe candidate for in vitro BMSCs gene delivery and had potential for in future in vivo gene therapy.

Keywords
Poly(amidoamine); Bone marrow stromal cell; Gene delivery; Biocompatibility; Cytotoxicity; Apoptosis
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Cytotoxicity and in vivo tissue compatibility of poly(amidoamine) with pendant aminobutyl group as a gene delivery vector
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 16, June 2010, Pages 4467–4476
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us