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The effect of strain rate on the precision of penetration of short densely-packed microprojection array patches coated with vaccine

Paper ID Volume ID Publish Year Pages File Format Full-Text
9011 614 2010 11 PDF Available
Title
The effect of strain rate on the precision of penetration of short densely-packed microprojection array patches coated with vaccine
Abstract

If skin's non-linear viscoelastic properties are mechanically exploited for precise antigen placement, there is tremendous promise for improved vaccines. To achieve this, we designed a Nanopatch™—densely packed micro-nanoprojections (>20,000/cm2) to directly deposit antigen to large numbers of epidermal Langerhans cells and dermal dendritic cells. Here, we controllably applied our Nanopatches™ with discrete conditions between peak strain rates of ∼100 s−1–7000 s−1 and quantified resulting penetration depths, delivery payloads and skin mechanics. Increasing the strain rate of application, we overcame key skin variability, achieving increases in both projection penetration depth (by over 50% length) and area coverage of a full array (from 50% to 100%). This delivery depth precision opens the way for more fully utilizing the skin's immune function. Furthermore, we yielded new insights on mechanical behaviour of skin, including: 1) internal skin property changes that could affect/facilitate penetration; 2) projection design to dictate penetration depth; 3) puncture mechanics of skin in this strain rate range. Indeed, we show delivery of a model vaccine using our tested range of strain rates achieved functionally relevant tunable systemic antibody generation in mice. These findings could be of great utility in extending skin strata vaccine targeting approaches to human use.

Keywords
Skin; Drug delivery; Mechanical properties; Vaccine; Microneedles
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The effect of strain rate on the precision of penetration of short densely-packed microprojection array patches coated with vaccine
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 31, Issue 16, June 2010, Pages 4562–4572
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us